In order to conduct more relevant experiments regarding the association between IFN-α and hepatitis infection, Yang et al (68) established a hepatoma cell line; cells were isolated from the liver tumor tissue of a male patient with chronic HCV infection (Hunan Provincial Tumor Hospital, Changsha, China) to create the HLCZ01 cell lines, which was the first cell line to support the entire life cycle of HBV and HCV. This evidence concerns the gene IFNA1 and hepatocellular carcinoma.