Moreover, the proportions of peripheral FoxP3+ Treg, Nrp1+ Helios+ thymic Treg, or LAG3+ CD49b+ Tr1 cells in the absence of IL-10 (i.e., between 4× WT and 4× KO mice) were not significantly different (data not shown), supporting our above-described observations which showed a lack of evidence for conventional CD4+ Treg cells as a cause of hyporesponsiveness in our infection model. The gene discussed is LAG3; the disease is infection.