This chromosome 7p gain has been associated with lymph node metastasis in lung cancer (29) and a detailed analysis of chromosome 7 identified various regions of alteration (30), including EGFR. Although gains of chromosome 7 result in an increase in the copy number of various genes located on this chromosome, data from the present study indicate that BRAF may also represent a target for its selection and clonal progression (19). This evidence concerns the gene BRAF and metastatic malignant neoplasm in the lymph nodes.