Similar to the results obtained from the IHC, western blotting identified TIGAR overexpression across a panel of six NPC cell lines of varying differentiation statuses (HK-1 was from differentiated from NPC; HONE-1, HONE-1-LMP1, HONE-1-EBV and CNE2 were from poorly-differentiated NPC; and C666-1 was from undifferentiated NPC) compared with the normal NP460 hTert nasopharyngeal cell line and the normal Het-1A esophageal epithelial cell line (Fig. 1D). Here, TIGAR is linked to nasopharyngeal carcinoma.