PIK3CA and thyroid gland carcinoma: Additionally, we also found a novel mutation in gene PIK3CA (c.3001C>G, p.L1001V), which the PolyPhen-2 and SIFT software predicted as a deleterious mutation; interestingly, we found a previous study which reported a different mutation of c.3001C>A in thyroid carcinoma [40], which may suggest that this is a hot spot for mutations in different carcinomas.