As shown in Fig. 1D, the ATP1A1 p12T-ins minigene construct (testing the 12T-insertion allele conferring protection to high blood pressure) exhibited significantly lower transcriptional activity than the p12T-del minigene construct (12T-deletion allele associated with high blood pressure) in the three cell lines tested (in Cos1, P < 0.05; in HEK293 and MDA-MB-468, P < 0.01). This evidence concerns the gene ATP1A1 and hypertensive disorder.