To validate the strong association between WHO meningioma grade and both PD-L1 mRNA and protein expression we used a separate cohort (TMA310), and analyzed staining of the same RNAscope probe (Fig. 4A) and two different IHC antibodies – the same PD-L1 Sinobiological antibody used for IHC of TMAs 283 & 285 (Fig. 4B) and an additional PD-L1 antibody (405.9A11) (Fig. 4C). This evidence concerns the gene CD274 and meningioma.