Scoring the numbers of signal spots per cell in the same two TMAs (TMA283 & 285) using the open source software CellProfiler analysis, we found similar results – that PD-L1 mRNA was highly expressed in 33.8% of WHO grade I, 70.3% WHO grade II, and 71.4% WHO grade III meningioma (Fig. 3D and S5B). The gene discussed is CD274; the disease is meningioma.