Furthermore, the downregulation of EphA5 expression could be reversed in all 6 PCa cell lines by treating cells with the DNA demethylating agent 5-aza-2′-deoxycytidine, implying that hypermethylation was an important reason for promoting the downregulation of EphA5 expression in prostate cancer. The gene discussed is EPHA5; the disease is Familial prostate cancer.