On univariate analyses, the three biomarkers and four clinicopathological factors (histologic grade, regional lymph nodes, TNM classification and therapy) were all confirmed as prognostic factors for OS, while only myosin-9 and three clinicopathological factors (histologic grade, regional lymph nodes and TNM classification) were risk factors for tumour recurrence of ESCC. This evidence concerns the gene MYH9 and esophageal squamous cell carcinoma.