CCND1 and neoplasm: By immunohistochemistry, we have revealed a significant increase in the expression of β-catenin and two known downstream targets of Wnt/β-catenin pathway cyclin D1 (CCND1) and Axin2 in the tumor tissues of the ApcMin/+;Slit2 and DMH/DSS-Slit2 mice, as opposed to their respective controls (Figure 4A, and Supplementary Figure 5A).