Further analysis of the tumor tissues from the ApcMin/+;Slit2 mice and DMH/DSS-Slit2 mice revealed that activation of Slit2/Robo1 signaling led to an increased pSrc (Tyr 416) in these tumor tissues compared with their respective controls (Figure 5A and Supplementary Figure 6B). This evidence concerns the gene ROBO1 and neoplasm.