DCLK1 and renal cell carcinoma: In order to assess the therapeutic potential of silencing DCLK1 in RCC we transfected Caki-2 cells with 25 nM DCLK1 siRNA (siDCLK1) targeting the primary DCLK1 protein-encoding transcripts (Fig S3A) or 25 nM siRNA of a scrambled sequence targeting no known genes (siSCR) as a control for 48 or 72 h.