Analysis of 30 primary lung cancer tissues stained with MAb 54-1 revealed an overall positivity for brachyury in 19/30 primary lung tumors, including 1/5 (20%) adenocarcinoma, 3/4 (75%) bronchioloalveolar carcinomas, 3/3 (100%) large cell carcinomas, 2/3 (67%) adenosquamous carcinomas, and 9/13 (69%) squamous cell carcinomas (Fig. 4D). Here, TBX1 is linked to adenosquamous carcinoma.