In the context of clinical studies of brachyury-based vaccines, we believe MAb 54-1 could be of potential use to determine what type of tumors express brachyury and could therefore be targets, and to evaluate the presence of brachyury-positive tumor cells pre vs. post-treatment, therefore assisting in the interpretation of the efficacy of brachyury-based therapies. This evidence concerns the gene TBX1 and neoplasm.