While our group and others have previously employed various polyclonal [7, 8, 17] or murine monoclonal [13, 16] antibodies (MAb) to evaluate the expression of brachyury in a variety of human tissues and in human tumor cell lines, we have found that the specificity and/or affinity of those antibodies are relatively low when evaluated in western blot and ELISA assays. Here, TBX1 is linked to neoplasm.