Regarding research studies of EMT, MAb 54-1 could be used for the detection of brachyury protein, concurrently with other epithelial and mesenchymal markers, either in human tumor cell lines in vitro or in vivo growing as xenografts, or in immunohistochemistry studies of primary and/or metastatic tumor lesions. This evidence concerns the gene TBX1 and metastatic neoplasm.