GFAP and brain disorder: Secondly, we detected histopathological markers related to pathogenesis of brain diseases such as the ionized calcium-binding adaptor molecule (Iba-1, microglial marker upregulated during activation of these cells), glial fibrillary acidic protein (GFAP, astroglial marker), Fluoro-Jade B (marker of neurodegeneration) and Hoechst 33258 (marker of cell nuclei).