In conclusion, even if our study has some limitations as using of bioimpendance method to measure body fat distribution, the results suggest that PROX1 variant has a pleiotropic effect on type 2 diabetes mellitus risk, and the rs340874 C/T PROX1 SNP is associated with NEFA/glucose metabolism alterations rather than defects of insulin secretion. This evidence concerns the gene PROX1 and type 2 diabetes mellitus.