Corroborating our in vitro experiments mimicking the E0771 tumor microenvironment of obese mice, where we demonstrated that leptin is not produced by the tumor cells or macrophages but by obese adipocytes, overall our in vivo results indicate that in this DIO tumor model, when mice are fed 33% HFD, leptin inhibition at the doses used was not enough to significantly reduce tumor growth, particularly when the tumor-promoting factors CCL2 and IL-6 are present. The gene discussed is LEP; the disease is neoplasm.