NOTCH3 and neoplasm: Interestingly, our results suggest that macrophages are more responsive than E0771 cells to the action of leptin on Notch 3 expression at lower levels of leptin, since 0.62 nM (10 ng/mL) leptin already upregulate Notch 3 in macrophages whereas a concentration of 6.2 nM (100 ng/mL), 10-fold higher, is required in E0771 cells to see a consistent increase in Notch 3 expression in these tumor cells [54].