In two independent experiments, lentiviral-based expression of truncated CD22ΔE12–14 protein (but not FL CD22) in CD22ΔE12-negative Burkitt's leukemia/lymphoma cell line DAUDI resulted in a marked increase in the size of day 7 colonies on consistent with increased self-renewal as well as increase of its in vitro clonogenicity, as measured by increased number (per 100,000 cells) of colonies formed in semisolid methylcellulose cultures (421 ± 83 vs. 104 ± 40, linear contrast P-value = 0.0006) (Fig. 5C & D). This evidence concerns the gene CD22 and lymphoma.