To test whether PTEN is involved in the regulation of Akt activation by cathelicidin, we observed that phosphorylation of PTEN at Ser380, Thr382 and Thr383 in cultured colon cancer cells could be induced by LL-37 exposure (Fig. 7A) or in cancer cells in the cancer animal models (Fig. 7C). This evidence concerns the gene AKT1 and colonic neoplasm.