Similarly, PTEN loss and B-Raf(V600E) expression following Nkx3.1-driven TAM-inducible Cre expression (Nkx3.1CreERT2Ptenlox/loxB-RafV600E/+) resulted in murine prostate tumors that were inherently castration-resistant and which metastasized to the lung, lymph nodes and bone marrow [128]. This evidence concerns the gene PTEN and prostate neoplasm.