Screening a panel of prostate cancer cell lines for the expression of the five ETS genes described to be involved in genomic rearrangements in PCa (ERG, ETV1, ETV4, ETV5 and FLI1), we found two cell lines – MDA-PCa-2b and PC3 – showing co-overexpression of ETV1 and ETV4. In fact, a similar profile was reported by Hollenhorst et al. for PC3 cells [27], but ETV1 was not considered as overexpressed and the MDA-PCa-2b cell line was not evaluated. This evidence concerns the gene ETV4 and Familial prostate cancer.