Furthermore, in vitro IKK kinase assay revealed that activation of the IKK kinase complex induced by TNF-α treatment was prolonged in miR-500–overexpressing cells, but was rapidly decreased in miR-500–inhibited cells (Figure 5G), suggesting that miR-500 overexpression sustains NF-ĸB activation in gastric cancer cells. The gene discussed is TNF; the disease is gastric cancer.