To investigate the possibility that AKT phosphorylation enhances the ability of TBX3 to promote migration we next compared the impact of WT TBX3 and the TBX3 S720A and TBX3 S720E mutants on the migratory ability of the WM1650 RGP melanoma cells using an in vitro cell motility assay. Here, AKT1 is linked to melanoma.