Due to batch-to-batch variations of hrEFEMP1 production, which hindered the study of signaling pathways affected by EFEMP1, we made a lentiviral vector that expresses ectopic EFEMP1 (with or without an N-terminal FLAG tag), and infected multiple human high-grade-glioma-derived cell lines (U251, U87, LN229), and a glioblastoma-derived primary culture (G43-SA). Here, EFEMP1 is linked to glioma.