Fig. 6 shows that inclusion of ADD3 exon 14 is favored in FECD corneal endothelial samples (average Ψ = 0.32), but this exon is almost always excluded in samples from controls (average Ψ = 0.05). Once again, the splicing pattern for exon 14 of ADD3 in the corneal endothelium from an FECD patient who does not have a repeat expansion (FECD 038) resembles that from the control samples (Ψ = 0.07). RT-PCR analysis of this splicing event (Fig. 6B) confirmed the increased inclusion of exon 14 (380-bp band) in FECD samples from patients who have TNR expansions. The gene discussed is ADD3; the disease is Fuchs endothelial corneal dystrophy.