To verify that overexpression of ATDC promotes activation of β-catenin in AKC cells in a manner similar to that seen in human PDA cells, we performed coimmunoprecipitation experiments and demonstrated that ATDC interacted with Dvl-2 in AKC14 and AKC17 cells, while knockdown of ATDC significantly inhibited the interaction of ATDC and Dvl-2 as well as β-catenin expression (Supplemental Fig. 14A). This evidence concerns the gene DVL2 and Patent ductus arteriosus.