The low-frequency variants at FAM134B (c.G1145C, p.S382T; transcript accession NM_001034850), NPC1L1 (c.C2920T, p.P974S) and IKZF1 (c.G1009A, p.G337S; transcript accession NM_006060) were all associated with lower risk for asthma, whereas the variants in SLC26A3 (c.A241G, p.I81V) and GSDMB (c.A365G, p.E122G; transcript accession NM_001042471) were associated with increased risk for asthma. This evidence concerns the gene NPC1L1 and asthma.