We also noticed that Akti-1/2 treatment increased background eIF2αP in eIF2αP-proficient cells consistent with our interpretation that Akt inhibition relieves the negative regulation of eIF2αP (Figure 6c, lane 3).35 When the biological effects of Akti-1/2 were tested, we found that Akt inhibition further enhanced the death of eIF2αP-proficient HT1080 or A549 tumor cells in response to H2O2 (Figures 6d and e). Here, AKT1 is linked to neoplasm.