Taken together, these data indicate that IFNα is significantly elevated in the supernatant and lysate of AI-resistant MCF-7:5C breast cancer cells and that activation of the canonical IFNα/IFNAR signaling pathway plays a critical role in driving the constitutive overexpression of IFITM1 and other ISGs in the resistant cells. The gene discussed is IFITM1; the disease is breast cancer.