To determine whether HIF-1 binds at this site, chromatin immunoprecipitation (ChIP) assays were performed in HCC-1954 breast cancer cells, which demonstrated hypoxia-inducible binding of HIF-1α and HIF-1β, but not HIF-2α, at this site (Fig. 1F). The gene discussed is HIF1A; the disease is hepatocellular carcinoma.