In response to hypoxia, CTGF mRNA expression increased in MCF-7 and MDA-MB-435 cells, PAI-1 mRNA expression increased in MCF-10A, MCF-7 and HCC-1954 cells, whereas Survivin mRNA expression increased in all five breast cell lines (Fig. 2A). This evidence concerns the gene CCN2 and hepatocellular carcinoma.