To determine if Gli1 participated in the induction of EMT in NSCLC cells, phase contrast microscopy was used to assess the morphology of A549 cells 24 h subsequent to si-Gli1, si-VE or blank transfection, and 18 h subsequent to TGF-β1 stimulation (Fig. 4A). This evidence concerns the gene TGFB1 and non-small cell lung carcinoma.