Similar results were obtained in the other NSCLC cell lines, with 5 ng/ml TGF-β1-treatment of H460 and SK-MES-1 cells for 48 h resulting in increased expression levels of vimentin but a reduction in E-cadherin expression, compared with untreated cells (Fig. 1C and D). This evidence concerns the gene VIM and non-small cell lung carcinoma.