CDK2 and infection: In contrast, infection of Cdk2-SN-NeoR rAAV plus transfection of pX330 CRISPR/Cas9 plasmid co-expressing a Cdk2 guide strand RNA (gCdk2) that targets a unique sequence between exons 3 and 4 resulted in an impressive 41/48 (85%) NeoR colonies containing recombined Cdk2-SN (Figure 2B and C) (Supplementary Figure S2D).