CDK2 and infection: Based on locus and integration specific PCR assays, infection of RPE cells with only Cdk2-SN-NeoR rAAV (no CRIPSR) resulted in 2/26 (<8%) NeoR colonies containing recombined Cdk2-SN single alleles, and no double alleles (Figure 2A) (Supplementary Figure S2A, B and E).