For example, a previous study demonstrated that the frequency of S100P hypomethylation did not differ significantly between pancreatic and breast cancer cell lines, whereas the frequency of hypomethylation of trefoil factor 2 (TFF2) and lipocalin 2 genes was significantly lower in the breast cancer cell lines than in pancreatic cancer cell lines (22). This evidence concerns the gene TFF2 and familial pancreatic carcinoma.