After the challenge infection with T. spiralis ML, the mice immunized with the rTspst displayed a 45.7% reduction in their intestinal adult worms (Figure 6) compared with the groups vaccinated with PBS alone; further, the reduction of intestinal adult worms between the immunized and adjuvant or MBP tag groups was statistically significant (F = 44.097, P < 0.05). This evidence concerns the gene MBP and infection.