APAF1 and early-onset autosomal dominant Alzheimer disease: Based on NCBI database, the screened differentially expressed genes Apaf1, Bace2, and Plcb4 were enriched in the “Alzheimer's disease-reference pathway” (P < 0.01) (Figure 4) and meanwhile significantly downregulated in Aβ25–35-injured PC12 cells after ME intervention as compared with Aβ25–35 group (P < 0.05) (Table 3).