MICAL1 and melanoma: By Annexin-V/PI flow cytometry assay, we also found that Mical1 depletion in these melanoma cells increased both early (annexin-V+PI−) and late (Annexin-V+PI+) apoptosis, compared with the effects seen in cells transfected with control siRNA (siScr, Fig. 6B), suggesting that Mical-1 protects BRAFV600E melanomas from apoptosis.