Annexin-V/PI staining assays indicated that Sema6A depletion induced apoptosis, associated with caspase 3/7 activation, in BRAFV600E clones 2/56 and 2/59 and in melanoma cell line 10538, with effects already evident at 24-36h (Fig. S4). The gene discussed is CASP3; the disease is melanoma.