To enhance the diagnostic value of PKM2/TRIM35 expression for early recurrence of HCC, we further performed a receiver-operating characteristic (ROC) curve analysis using the IHC data from cohort 4 to determine the efficacy of PKM2 and TRIM35 expression in discriminating early recurrence (less than 3 years) in patients with HCC. This evidence concerns the gene PKM and hepatocellular carcinoma.