PKM and cancer: In addition, a multivariate statistical analysis revealed that HCC patients with the signature of TRIM35 (−)/PKM2 (+) expression harbored a 2.25-fold higher risk of cancer recurrence (95% CI: 1.37–3.71, P < 0.001) and a 2.1-fold higher risk of death (95% CI: 1.19–3.71, P < 0.001) in the primary cohort (Table 3).