Kaplan–Meier analysis showed that the median overall survival (OS) time was 40.3 (95% CI: 33.3–47.4) months for patients with HCC who were positive for PKM2 and 60.8 (95% CI: 56.9–64.6) months for patients with HCC who were negative for PKM2 (P < 0.0001, log rank test, Figure 2A). This evidence concerns the gene PKM and hepatocellular carcinoma.