To characterize the oncogenic ability of NTKL, full-length NTKL was cloned into expressing vector pCDNA3.1 and stably transfected into HCC cell lines QGY-7703 and MHCC-97H cells (NTKL-7703 and NTKL-97H). The gene discussed is SCYL1; the disease is hepatocellular carcinoma.