Reduced PHGDH expression decreased phosphoserine levels in PHGDH amplified BT-20 cells, while nontumorigenic breast cancer epithelial MCF-10a cells did not require PHGDH for growth, exhibit alterations in glycolysis on shRNA knockdown of PHGDH, or show detectable labeling of phosphoserine from glucose [8]. This evidence concerns the gene PHGDH and breast cancer.