Although the impact of DLEC1 methylation on RFS was less evident than depth of invasion, lymphatic invasion, venous invasion, Lauren classification, and lymph node metastasis, the risk of recurrence in patients with higher DLEC1 methylation was still 2.43 times higher than those with lower DLEC1 methylation (P = 0.025) (Table 3). The gene discussed is DLEC1; the disease is metastatic malignant neoplasm in the lymph nodes.