Considering that obesity is implicated in eliciting ER stress and that inflammatory parameters were not overtly increased (Figure 1D,E), we assessed whether the hepatic tissue was undergoing ER stress by verifying whether differential splicing occurred at the XBP-1 gene or eIF2-alpha was being activated by phosphorylation. Here, EIF2S1 is linked to obesity due to melanocortin 4 receptor deficiency.