Regardless, we set up the experiments similarly to previously described system by adoptively transferring polyclonal naïve CD44lo Thy1.2+ TGF-βRIIf/f Lck-cre− (WT) or TGF-βRIIf/f Lck-cre+ (KO) CD4 T cells into congenic Thy1.1+ OT-II recipient mice and 1 day later infected mice with influenza WSN-GP33/66. Here, CD4 is linked to influenza.