For these reasons, we chose to exploit our established fluorescence-based RTM screening system in order to identify functional AS RNAs from a library of randomly generated AS RNAs that could increase the trans-splicing efficiency of a given RTM specific for the COL7A1 gene, which is associated with the devastating skin disease dystrophic epidermolysis bullosa (DEB) [16,17]. This evidence concerns the gene COL7A1 and Dystrophic epidermolysis bullosa.