MSP is a sensitive and specific method for detecting DNA methylation in tumor samples, and allowing the rapid examination of multiple samples [16], which revealed that the ADAMTS18 gene promoter was hypermethylated in 80% of the ccRCC-derived cell lines (with the exception of the 786-O cell line) and 43.6% of the ccRCC primary tumors. The gene discussed is ADAMTS18; the disease is nonpapillary renal cell carcinoma.