To test this, mutations were constructed in P. mirabilis HI4320, in the same glycolysis genes tested for E. coli: pgi, pfkA, tpiA, and pykA. Unexpectedly, any mutation that disrupted glycolysis in P. mirabilis resulted in a significant fitness defect using the same model of ascending infection (Fig. 2B). Here, PFKM is linked to infection.