The cytotoxicity of the doxazosin/IFN-α or IFN-γ combination on carcinoma cells were evaluated using fluorescence activated cell sorting (FACS) with the FITC-labeled Annexin V. As presented in Fig. 2C, pre-treatment with IFN-α or IFN-γ dramatically promoted doxazosin-induced apoptotic cell death, showing that IFN-α or IFN-γ enhances doxazosin-mediated cell growth inhibition. This evidence concerns the gene ANXA5 and carcinoma.