ESR1 and neoplasm: The object-oriented approach allows both a detailed analysis of each individual lobule and an image-wide analysis of the spatial relationships between lobules and tumor, and enables analysis of the lobules at high resolution, for example for ER/PR positivity, in acceptable processing time, for example enabling overnight batch processing of 10-12 WSI slides at 20× magnification on a normal PC with current processor technology, and correspondingly higher throughput on work stations with higher computational power.