We found that concentrations of 3-NP sufficient to selectively decrease respiration in cells expressing low TRAP1 levels, i.e. shTRAP1 neoplastic cells and mock-transfected MEF cells, were also effective in blocking the increase in mitochondrial superoxide and in cell death elicited by serum and glucose depletion; notably, 3-NP was ineffective in cells harboring high TRAP1 levels (mock cancer cells and TRAP1-overexpressing MEF cells) undergoing starvation (Fig. 3A-D, S2A, S2B). This evidence concerns the gene TRAP1 and cancer.