Accordingly, we found that knocking-down TRAP1 expression in human osteosarcoma SAOS-2 cells and human cervix carcinoma HeLa cells (Fig. 1A, 1B; cells expressing short hairpin TRAP1 RNAs were dubbed shTRAP1), caused a constitutive increase in the levels of intracellular ROS and of mitochondrial superoxide anion (Fig. 1D, 1E, 1G, 1H). Here, TRAP1 is linked to osteosarcoma.