NDE1 and microcephaly: Nde-1, the protein product of the centrosomal gene, mutated in microcephaly, has previously been shown to interact with CENP-F.22–26 We, therefore, investigated, by whole exome sequencing, a large cohort of patients (n=1000) with microcephaly (MCPH) for CENPF mutations, where no deleterious or potentially pathogenic variants were identified in known MCPH genes (CEP135, ASPM, WDR62, MCPH1, CEP152, STIL, CEP63 and CENPJ).