Overall, several of our results suggest that p-eIF4E plays a key role during MNV1 replication: MNV replication is impaired following disruption of the eIF4E-eIF4G interaction; blocking the signaling pathways leading to eIF4E phosphorylation inhibits replication; and phosphorylated eIF4E relocates to polysomes during infection. Here, EIF4E is linked to infection.