Among the other regions examined, we found that tumour methylation at the ITGB2 and mir10B loci showed no difference with matched benign tissue, the APC locus showed variable differences between tumour and matched benign and the remaining four loci (RARB, C5orf4 (FAXDC2), TACC2 and DGKZ) showed increased methylation in tumour vs matched benign samples, although to a lesser extent than the HES5 locus (Fig. 1b, c, d and Supplementary Figure 1f, g, h, i, j, k, l, m). This evidence concerns the gene HES5 and neoplasm.