HES5 and neoplasm: These consistent methylation changes at the HES5 promoter appear to be locus specific, as highlighted by the similarity of global methylation profiles (Supplementary Figure 1b, c, d and e) and the absence of consistent changes in DNA methylation at other genomic loci across spatially separated tumour samples from the same patient (Supplementary Figure 2, see section on supplementary data given at the end of this article).