Since a strong TA-CIN-specific CD4+ T cell response was observed after vaccination of naïve BALB/c mice with TA-CIN/GPI-0100 (Fig. 2B), we examined whether this TA-CIN-specific CD4+ T cell response was also induced in TC-1 tumor-bearing C57BL/6 mice after vaccination using both fresh and frozen vaccine. The gene discussed is CD4; the disease is neoplasm.