Importantly, mice vaccinated with TA-CIN formulated with GPI-0100 in mannitol solution and stored at −80°C generated similar TA-CIN-specific CD4+, CD8+ T cell and HPV16-specific neutralizing antibody responses to freshly formulated TA-CIN with GPI-0100 (Fig. 2). Here, CD4 is linked to cervical squamous intraepithelial neoplasia.